Cell-free protein synthesis and interferon action: protein kinase(s) and an oligonucleotide effector, pppA2'p5'A2'p5'A.
نویسندگان
چکیده
منابع مشابه
Increased nuclease activity in cells treated with pppA2'p5'A2'p5' A.
A series of 2'-5'-linked oligo(adenylic acid) triphosphate (2'-5' A) inhibitors of protein synthesis were described recently. These inhibitors are synthesized from ATP by an enzyme activated in interferon-treated cell extracts or rabbit reticulocyte lysates by double-stranded RNA. We show here that 2'-5' A is a potent inhibitor of protein synthesis in intact cells of different origin (human, mo...
متن کاملAn interferon-induced phosphodiesterase degrading (2'-5') oligoisoadenylate and the C-C-A terminus of tRNA.
A phosphodiesterase characterized by a generally higher activity on 2'-5' than on 3'-5' phosphodiester bonds was isolated from mouse L cells treated with interferon. A similar enzyme was purified from mouse reticulocytes. The phosphodiesterase 2'-PDi splits the 2'-phosphate bond of pppA2'p5'A2'p5'A, the oligonucleotide activator of ribonuclease F. The level of phosphodiesterase 2'-PDi is increa...
متن کاملChemical Modification Potentiates the Biological Activities of 2 - 5 A and Its
Chemical modification of p5’A2’(p5’A2’),p5’A by a periodate oxidation/Schiff base formation/borohydride reduction cycle gave a series of 2-5A analogues in which the ribose of the 2’-terminal nucleotide was transformed to an N-substituted morpholine (azahexapyranose). 2’,5’-Oligoriboadenylate 5’-monophosphates bearing this modification were 5-10 times more potent as antagonists of the action of ...
متن کاملTargeting RNA for degradation with a (2'-5')oligoadenylate-antisense chimera.
Antisense oligonucleotides hold considerable promise both as research tools for inhibiting gene expression and as agents for the treatment of a myriad of human diseases. However, targeted destruction of RNA has been difficult to achieve in a versatile, efficient, and reliable manner. We have developed an effective strategy for cleaving unique RNA sequences with 2-5A-dependent RNase, an endoribo...
متن کاملThe 100-kDa 2',5'-oligoadenylate synthetase catalyzing preferentially the synthesis of dimeric pppA2'p5'A molecules is composed of three homologous domains.
The 2-5A synthetases represent a family of proteins implicated in the mechanism of the antiviral action of interferon. When activated by double-stranded RNA, these proteins polymerize ATP into 2'-5'-linked oligomers with the general formula pppA(2'p5'A)n, n >/= 1. Three forms of human 2-5A synthetases have been described corresponding to proteins of 40/46 (p40/p46), 69/71 (p69/p71), and 100 kDa...
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ورودعنوان ژورنال:
- Haematology and blood transfusion
دوره 23 شماره
صفحات -
تاریخ انتشار 1979